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MicroSlicer™ Zero 1N
The MicroSlicers™ allow to cut sections of soft tissues like brain, liver or similar difficult-to-section material. The MicroSlicers™ are well known for their quality and superior performance, enjoying an excellent reputation in the neuroscience research community.
The MicroSlicers™ have a unique design for the vibratory motion of the cutting blade. To generate the shuttling blade motion in the MicroSlicer™, a smooth motorized system was adopted that is less prone to vertical vibration, compared to the traditional electromagnetic vibrating system. Traditional electromagnetic system is limited to a set constant vibration frequency of the blade. In contrast, the MicroSlicer™ allows adjustment to the shuttling frequency of the blade depending on the properties of the specimen. Thus, the MicroSlicer™ can cut thinner sections more easily and efficiently.
Objective of the MicroSlicer™
The MicroSlicer™ is an instrument designed to cut sections of animal or plant tissues, fresh or fixed, without freezing them.
Various fields of neuroscience, histochemistry and cytochemistry require incubation of tissues in different incubation media. Large tissue blocks and thick sections often end up with non-specific deposits of reaction products and areas of no reaction in the center of the specimen. This is due to poor penetration of the reaction media into the tissue. For this reason, thinner sections are preferred to perform histochemical and cytochemical reactions.
The simplest method to cut tissue sections is to cut frozen in a cryostat. However, it is well known that both cellular fine structure and enzyme activity suffer greatly from freezing and thawing. Nonfrozen sections are therefore preferred for histochemistry and cytochemistry. In recent years, rat or cat fresh brain sections (200 to 500 micrometers in thickness) have been used in neurophysiological and pharmacological research. In addition, tissue sections are used in a variety of studies in the field of botany.
Traditionally, non-frozen tissue sections have been cut by hand with a razor blade or by using microtomes or tissue choppers. However, these methods have various drawbacks, such as the irregular thickness of tissue sections, difficulty of obtaining thin sections, or extremely slow sectioning speed. The MicroSlicers™ are instruments for preparing non-frozen tissue sections easily and efficiently.
The MicroSlicer™ vibratory tissue slicer systems feature a fixed cutting amplitude and an adjustable frequency. Even the entry level model, DTK-1000N, comes equipped with an auto-reverse mechanism and detachable bath. In addition to that, the Zero 1N has a stepping motor for vertical movement of the specimen, as well as a retraction function. The Zero 1N model features the MicroSlicer™ Zero-Z technology with a redesigned cutting head for dramatically reduced Z-axis vibration. The layout of the switches on both sides of the tray allows for easy operation and reduced spillage on the controls.
Applications: Neuroscience Research, Physiology, Immunocytochemistry, Pharmacology, Pathology, Fluorescent Antibody Study, Histology, Cytology
MicroSlicer™ Accessories and Consumables
MicroSlicer™ Zero 1N
the first vibratory slicer with Zero Z technology
This model MicroSlicer™ with Zero Z technology dramatically reduces the Z-axis vibrations to approximately 2.5 microns. It is also equipped with a specimen retraction system. The specimen advance is manual, but the specimen can be moved up or down by a motorized button. A function switch can stop, advance or retract the cutting action, saving valuable time.
Functions Include:
| 1. Frequency dial 2. Cutting speed dial 3. Function buttons; reverse; stop; forward 4. Power button 5. Retraction button 6. Specimen up/down dial |
7. Raise specimen button 8. Lower specimen button 9. Blade holder 10. Specimen tray 11. Ice bath |
MicroSlicer™ Specifications Overview
| Model | Prod. No. 10000N and 10000N-220 MicroSlicer™ DTK-1000N |
Prod. No. 10111N and 10111N-220 MicroSlicer™ Zero 1N |
| Electrical | 115V or 230V, 50/60Hz | 115V or 230V, 50/60Hz |
| Blade Oscillating Frequency | 0~50Hz | 0~50Hz |
| Blade Oscillating Width | 2mm fixed | 2mm fixed |
| Cutting Speed | 0-68/mm/min | 0-68/mm/min |
| Reverse Speed | 104mm/min | 104mm/min |
| Maximum Specimen Height | 20mm | 20mm |
| Specimen Retraction Function | no | yes |
| Maximum Specimen Size | 25 x 30mm | 25 x 30mm |
| Specimen Advance | Manual | Motorized |
| Blade Holder Size | For 30mm blade | For 30mm blade |
| Automatic Operation | no | no |
The retraction mechanism is a function included in the Zero 1N model that prevents the retracting blade from touching the face of the specimen. The specimen tray is lowered by 120 micrometers after the cutting of each section and is raised by exactly the same distance when a new cutting cycle starts.
